Immunohistochemistry:Article Title: Coordination by Cdc42 of Actin, Contractility, and Adhesion for Melanoblast Movement in Mouse Skin
Article Snippet: .. Mouse anti-Cdc42, Mouse anti-GM130, Mouse anti-BrdU (for IHC), Mouse anti-ROCKII (BD Biosciences), Goat anti-DCT TRP2, Mouse anti-T-MLC MRCL3/MRCL2/MYL9 (E-4), Rabbit-antiWAVE2 (Santa Cruz), Mouse anti-Rac1 (Cytoskeleton), Mouse anti-vinculin (hVIN1, Sigma), Rabbit anti-Phospho-Myosin Light Chain 2 (Thr18/Ser19), Rabbit anti-Phospho Myosin Light Chain 2 (Ser19) for immunofluorescence (Cell Signalling Technology), Rabbit anti-phospho-paxillin, Rabbit antiGAPDH, Rabbit anti-α4 integrin for Western blots, Rabbit anti-β3 integrin, Rabbit anti-α6 integrin (Cell Signalling), Rabbit anti-α4 integrin (D2E1, Cell Signalling Technology, for immunofluorescence) rabbit anti-Ki67 (SP6, Neomarkers), Mouse anti-BrdU (Dako), Rabbit anti-p34-Arc, Rat anti-β1 integrin (Millipore), Mouse-anti GAPDH (Ambion) Rabbit anti-α-Tubulin, Rat anti-tyrosinated tubulin, mouse anti-FMNL2 antibody (Abcam) Mouse anti-Talin (8D4, Sigma), Sheep anti-MITF (kind gift of Claudia Wellbrock, Manchester University, UK). .. Monoclonal biotinylated rabbit anti-goat IgG secondary antibody was obtained from Dako.
Article Title: Coordination by Cdc42 of Actin, Contractility, and Adhesion for Melanoblast Movement in Mouse Skin
Article Snippet: .. Mouse anti-Cdc42, Mouse anti-GM130, Mouse anti-BrdU (for IHC), Mouse anti-ROCKII (BD Biosciences), Goat anti-DCT TRP2, Mouse anti-T-MLC MRCL3/MRCL2/MYL9 (E-4), Rabbit-antiWAVE2 (Santa Cruz), Mouse anti-Rac1 (Cytoskeleton), Mouse anti-vinculin (hVIN1, Sigma), Rabbit anti-Phospho-Myosin Light Chain 2 (Thr18/Ser19), Rabbit anti-Phospho Myosin Light Chain 2 (Ser19) for immunofluorescence (Cell Signalling Technology), Rabbit anti-phospho-paxillin, Rabbit antiGAPDH, Rabbit anti-α4 integrin for Western blots, Rabbit anti-β3 integrin, Rabbit anti-α6 integrin (Cell Signalling), Rabbit anti-α4 integrin (D2E1, Cell Signalling Technology, for immunofluorescence) rabbit anti-Ki67 (SP6, Neomarkers), Mouse anti-BrdU (Dako), Rabbit anti-p34-Arc, Rat anti-β1 integrin (Millipore), Mouse-anti GAPDH (Ambion) Rabbit anti-α-Tubulin, Rat anti-tyrosinated tubulin, mouse anti-FMNL2 antibody (Abcam) Mouse anti-Talin (8D4, Sigma), Sheep anti-MITF (kind gift of Claudia Wellbrock, Manchester University, UK). .. Monoclonal biotinylated rabbit anti-goat IgG secondary antibody was obtained from Dako.
Immunofluorescence:Article Title: Coordination by Cdc42 of Actin, Contractility, and Adhesion for Melanoblast Movement in Mouse Skin
Article Snippet: .. Mouse anti-Cdc42, Mouse anti-GM130, Mouse anti-BrdU (for IHC), Mouse anti-ROCKII (BD Biosciences), Goat anti-DCT TRP2, Mouse anti-T-MLC MRCL3/MRCL2/MYL9 (E-4), Rabbit-antiWAVE2 (Santa Cruz), Mouse anti-Rac1 (Cytoskeleton), Mouse anti-vinculin (hVIN1, Sigma), Rabbit anti-Phospho-Myosin Light Chain 2 (Thr18/Ser19), Rabbit anti-Phospho Myosin Light Chain 2 (Ser19) for immunofluorescence (Cell Signalling Technology), Rabbit anti-phospho-paxillin, Rabbit antiGAPDH, Rabbit anti-α4 integrin for Western blots, Rabbit anti-β3 integrin, Rabbit anti-α6 integrin (Cell Signalling), Rabbit anti-α4 integrin (D2E1, Cell Signalling Technology, for immunofluorescence) rabbit anti-Ki67 (SP6, Neomarkers), Mouse anti-BrdU (Dako), Rabbit anti-p34-Arc, Rat anti-β1 integrin (Millipore), Mouse-anti GAPDH (Ambion) Rabbit anti-α-Tubulin, Rat anti-tyrosinated tubulin, mouse anti-FMNL2 antibody (Abcam) Mouse anti-Talin (8D4, Sigma), Sheep anti-MITF (kind gift of Claudia Wellbrock, Manchester University, UK). .. Monoclonal biotinylated rabbit anti-goat IgG secondary antibody was obtained from Dako.
Article Title: Coordination by Cdc42 of Actin, Contractility, and Adhesion for Melanoblast Movement in Mouse Skin
Article Snippet: .. Mouse anti-Cdc42, Mouse anti-GM130, Mouse anti-BrdU (for IHC), Mouse anti-ROCKII (BD Biosciences), Goat anti-DCT TRP2, Mouse anti-T-MLC MRCL3/MRCL2/MYL9 (E-4), Rabbit-antiWAVE2 (Santa Cruz), Mouse anti-Rac1 (Cytoskeleton), Mouse anti-vinculin (hVIN1, Sigma), Rabbit anti-Phospho-Myosin Light Chain 2 (Thr18/Ser19), Rabbit anti-Phospho Myosin Light Chain 2 (Ser19) for immunofluorescence (Cell Signalling Technology), Rabbit anti-phospho-paxillin, Rabbit antiGAPDH, Rabbit anti-α4 integrin for Western blots, Rabbit anti-β3 integrin, Rabbit anti-α6 integrin (Cell Signalling), Rabbit anti-α4 integrin (D2E1, Cell Signalling Technology, for immunofluorescence) rabbit anti-Ki67 (SP6, Neomarkers), Mouse anti-BrdU (Dako), Rabbit anti-p34-Arc, Rat anti-β1 integrin (Millipore), Mouse-anti GAPDH (Ambion) Rabbit anti-α-Tubulin, Rat anti-tyrosinated tubulin, mouse anti-FMNL2 antibody (Abcam) Mouse anti-Talin (8D4, Sigma), Sheep anti-MITF (kind gift of Claudia Wellbrock, Manchester University, UK). .. Monoclonal biotinylated rabbit anti-goat IgG secondary antibody was obtained from Dako.
Western Blot:Article Title: Coordination by Cdc42 of Actin, Contractility, and Adhesion for Melanoblast Movement in Mouse Skin
Article Snippet: .. Mouse anti-Cdc42, Mouse anti-GM130, Mouse anti-BrdU (for IHC), Mouse anti-ROCKII (BD Biosciences), Goat anti-DCT TRP2, Mouse anti-T-MLC MRCL3/MRCL2/MYL9 (E-4), Rabbit-antiWAVE2 (Santa Cruz), Mouse anti-Rac1 (Cytoskeleton), Mouse anti-vinculin (hVIN1, Sigma), Rabbit anti-Phospho-Myosin Light Chain 2 (Thr18/Ser19), Rabbit anti-Phospho Myosin Light Chain 2 (Ser19) for immunofluorescence (Cell Signalling Technology), Rabbit anti-phospho-paxillin, Rabbit antiGAPDH, Rabbit anti-α4 integrin for Western blots, Rabbit anti-β3 integrin, Rabbit anti-α6 integrin (Cell Signalling), Rabbit anti-α4 integrin (D2E1, Cell Signalling Technology, for immunofluorescence) rabbit anti-Ki67 (SP6, Neomarkers), Mouse anti-BrdU (Dako), Rabbit anti-p34-Arc, Rat anti-β1 integrin (Millipore), Mouse-anti GAPDH (Ambion) Rabbit anti-α-Tubulin, Rat anti-tyrosinated tubulin, mouse anti-FMNL2 antibody (Abcam) Mouse anti-Talin (8D4, Sigma), Sheep anti-MITF (kind gift of Claudia Wellbrock, Manchester University, UK). .. Monoclonal biotinylated rabbit anti-goat IgG secondary antibody was obtained from Dako.
Article Title: Coordination by Cdc42 of Actin, Contractility, and Adhesion for Melanoblast Movement in Mouse Skin
Article Snippet: .. Mouse anti-Cdc42, Mouse anti-GM130, Mouse anti-BrdU (for IHC), Mouse anti-ROCKII (BD Biosciences), Goat anti-DCT TRP2, Mouse anti-T-MLC MRCL3/MRCL2/MYL9 (E-4), Rabbit-antiWAVE2 (Santa Cruz), Mouse anti-Rac1 (Cytoskeleton), Mouse anti-vinculin (hVIN1, Sigma), Rabbit anti-Phospho-Myosin Light Chain 2 (Thr18/Ser19), Rabbit anti-Phospho Myosin Light Chain 2 (Ser19) for immunofluorescence (Cell Signalling Technology), Rabbit anti-phospho-paxillin, Rabbit antiGAPDH, Rabbit anti-α4 integrin for Western blots, Rabbit anti-β3 integrin, Rabbit anti-α6 integrin (Cell Signalling), Rabbit anti-α4 integrin (D2E1, Cell Signalling Technology, for immunofluorescence) rabbit anti-Ki67 (SP6, Neomarkers), Mouse anti-BrdU (Dako), Rabbit anti-p34-Arc, Rat anti-β1 integrin (Millipore), Mouse-anti GAPDH (Ambion) Rabbit anti-α-Tubulin, Rat anti-tyrosinated tubulin, mouse anti-FMNL2 antibody (Abcam) Mouse anti-Talin (8D4, Sigma), Sheep anti-MITF (kind gift of Claudia Wellbrock, Manchester University, UK). .. Monoclonal biotinylated rabbit anti-goat IgG secondary antibody was obtained from Dako.
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